Day 11 part 1 (nee) micropollutant

Environmental Biotechnology

🌍 Micropollutants — The Invisible Invaders

Definition: Micropollutants are chemical compounds present in very low concentrations — typically micrograms (µg) per liter or even nanograms (ng) per liter of water. Despite their tiny amounts, they can have big environmental impacts.

Examples: These are substances we use daily — from household products, personal care items, pharmaceuticals, and cleaning agents — each with distinct chemical structures and functions.


🧴 Sources and Pathways

When we use these compounds, they enter wastewater systems through household drains or industrial runoff. 🚽 → 💧 → 🏭 (Wastewater Treatment Plant) → 🌊 (Environment)

However, conventional wastewater treatment plants are not designed to remove all micropollutants effectively. As a result, these compounds pass through and end up in rivers, lakes, and groundwater — where they can affect aquatic ecosystems and even return to us through drinking water.


⚗️ Why Micropollutants Are a Problem

Even at low concentrations, micropollutants can:

  • ☠️ Be toxic to aquatic organisms.
  • 🧬 Cause endocrine disruption (hormonal imbalances) in fish and other wildlife.
  • 🌱 Accumulate in the food chain, leading to bioaccumulation and biomagnification.
  • 🧠 Affect microbial communities, altering natural biodegradation processes.

They are chemically diverse — from antibiotics and hormones to pesticides and surfactants — which makes their removal especially challenging.


🔬 Degradation and Removal Mechanisms

Scientists study how these compounds can be removed or broken down. The main mechanisms include:

1. Biodegradation (Microbial Breakdown) 🧫

Microorganisms like bacteria can metabolize micropollutants, using them as a carbon or energy source. However:

  • Some compounds are recalcitrant (resistant to degradation).
  • Different bacteria specialize in degrading different compounds.
  • Environmental conditions (oxygen, nutrients, pH, temperature) affect the efficiency.

2. Abiotic Degradation

This includes chemical and physical processes like:

  • Photodegradation (sunlight-driven breakdown).
  • Hydrolysis (reaction with water).
  • Oxidation/reduction reactions.

3. Adsorption 🧲

Certain materials (like activated carbon) can trap micropollutants, removing them from water without necessarily breaking them down.


🧬 Identifying the Bacteria Involved

Researchers use molecular tools to find which bacteria degrade which compounds:

  • Stable isotope probing (SIP): tracks labeled carbon from the pollutant into microbial DNA.
  • Metagenomics & 16S rRNA sequencing: identifies microbial community composition.
  • Functional assays & transcriptomics: reveal active degradation pathways.

Each bacterial strain may have unique enzymes that can cleave specific chemical bonds — e.g. aromatic ring cleavage, hydroxylation, or dehalogenation.


🧪 Examples of Bacterial Diversity

Different bacterial species use different metabolic strategies to attack pollutants:

Compound TypeExample BacteriaMechanism
Phenolic compoundsPseudomonas spp.Hydroxylation → Ring cleavage
Chlorinated compoundsDehalococcoides spp.Reductive dechlorination
PharmaceuticalsSphingomonas, RhodococcusCo-metabolism or enzymatic oxidation

Each degradation pathway reflects adaptation and specialization — showing how microbial diversity is key to environmental resilience.


🌱 Summary of Key Ideas

ConceptKey Takeaway
MicropollutantsTiny concentrations, huge effects
Main sourcesHouseholds, pharmaceuticals, personal care, industry
Environmental impactToxicity, endocrine disruption, bioaccumulation
Removal mechanismsBiotic (microbial) + abiotic (chemical/physical)
Research focusIdentifying bacteria and degradation pathways
Bacterial diversityDifferent microbes = different enzymatic tools

Quiz

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